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Kisspeptin-10

Kisspeptin-10 (metastin 45–54, CAS 374683-24-6) is the minimal 10-amino acid C-terminal fragment of the kisspeptin-54 (metastin) peptide that retains full agonist potency at the kisspeptin receptor (KISS1R, formerly GPR54), with a molecular weight of approximately 1,302.4 Da. As the most potent endogenous activator of hypothalamic GnRH neurons, kisspeptin signaling is the master gatekeeper of the hypothalamic-pituitary-gonadal (HPG) axis. Kisspeptin-10 is an essential research tool in reproductive neuroendocrinology, puberty onset, and fertility regulation.

Technical Specifications

Property Value
CAS Number 374683-24-6
Molecular Formula C₆₃H₈₃N₁₇O₁₄
Molecular Weight ~1,302.4 Da
Purity ≥98% (HPLC-verified)
Appearance Lyophilized white powder
Solubility Soluble in sterile water and aqueous buffers
Storage -20°C (lyophilized), 2–8°C (reconstituted)

Mechanism of Action

Kisspeptin-10 binds with high affinity to KISS1R (GPR54), a Gαq/11-coupled GPCR expressed at high density on hypothalamic GnRH neurons in the preoptic area and arcuate nucleus. Receptor activation triggers PLC-mediated IP₃ and DAG generation, mobilizing intracellular calcium and activating PKC. Calcium-dependent exocytosis of GnRH from neurosecretory terminals in the median eminence delivers GnRH into the hypophyseal portal circulation, where it stimulates gonadotropin (LH and FSH) release from anterior pituitary gonadotrophs. This KISS1R→GnRH→LH/FSH cascade is the central regulatory axis controlling pubertal onset, ovulation, and gonadal steroidogenesis. Kisspeptin neurons in the arcuate nucleus (KNDy neurons: co-expressing kisspeptin, neurokinin B, and dynorphin) serve as the pulse generator for episodic GnRH/LH secretion, integrating feedback from sex steroids (estradiol and testosterone). Kisspeptin-10's compact size, high potency, and water solubility make it ideal for studying KISS1R pharmacology in vitro and pulsatile GnRH dynamics in vivo.

Research Applications

  • Primary: Reproductive neuroendocrinology — GnRH pulse generation, HPG axis regulation, KISS1R signaling
  • Secondary: Puberty onset mechanisms, fertility and ovulation research, kisspeptin neuron electrophysiology (KNDy neurons)
  • Model Systems: In vitro (KISS1R-transfected cell lines, GT1-7 GnRH neuronal cells), in vivo (LH pulse monitoring in rodents and sheep, kisspeptin microinjection studies, KISS1R knockout models)

Quality Control & Analytical Methods

  • HPLC: ≥98% purity verification at 214/220 nm (C18 reversed-phase column)
  • Mass Spectrometry: ESI-MS for molecular weight confirmation (~1,302.4 Da)
  • Peptide Content: Amino acid analysis (AAA) for net peptide content determination
  • Endotoxin: <1 EU/mg (LAL assay)
  • TFA Content: <1% (ion chromatography)

Stability & Storage

Condition Stability
-20°C (lyophilized) 24 months
4°C (lyophilized) 6 months
25°C (lyophilized) 1 month
Reconstituted (4°C) 7 days
Reconstituted (-20°C) 30 days

Key Research References

  • Seminara et al. (2003) — The GPR54 gene as a regulator of puberty. N Engl J Med. PMID: 14573733
  • de Roux et al. (2003) — Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proc Natl Acad Sci USA. PMID: 12944565
  • Clarkson et al. (2009) — Kisspeptin-GPR54 signaling is essential for ovulation and fertility. Endocrinology. PMID: 19137458

Source & Purchase

For researchers requiring research-grade KISSPEPTIN 10 with full analytical documentation including HPLC, LC-MS, and Certificate of Analysis, visit the HK Peptides product page for specifications, bulk pricing, and ordering.

View KISSPEPTIN 10 Product →

FAQ

Q: What purity level is standard for Kisspeptin-10? A: HK Peptides supplies Kisspeptin-10 at ≥98% purity by HPLC with full COA documentation.

Q: How should Kisspeptin-10 be stored for research use? A: Lyophilized Kisspeptin-10 should be stored at -20°C. Reconstituted solutions at 4°C for short-term use (up to 7 days).